440. RHODOSPIRILLACEAE MEDIUM (RsM)

Yeast extract 0.30 g

Na₂-Succinate 1.0 g

(NH₄)-Acetate 0.50 g

Fe(III)citrate solution (0.1% in H₂O) 5.0 ml

KH₂PO₄ 0.50 g

MgSO₄ × 7 H₂O 0.40 g

NaCl 0.40 g

NH₄Cl 0.40 g

CaCl₂ × 2 H₂O 0.05 g

Vitamin B₁₂ solution (10 mg in 100 ml H₂O) 0.40 ml

Trace element solution SL-6 (see below) 1.0 ml

L-Cysteine HCl 0.30 g

Resazurin (0,1%) 0.50 ml

Distilled water 1000.0 ml

Trace element solution SL-6:

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

H₃BO₃ 0.3 g

CoCl₂ × 6 H₂O 0.2 g

CuCl₂ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.