491. ARCHAEGLOBUS NEPTUNUS MEDIUM (ARCNM)

NH4Cl 0.33 g

KCl 0.33 g

CaCl2 x 6 H2O 0.33 g

KH2PO4 0.33 g

NaCl 18.0 g

MgCl2 x 6 H2O 4.33 g

NaHCO3 2.0 g

Resazurin 0.001 g

Na2S x 9 H2O 0.5 g

Trace element solution (see below) 1 ml

Vitamin solution (see below) 1 ml

Distilled water 1000 ml


Trace element solution:

(NH4)2Fe(SO4)2 x 6 H2O (Mohr's salt) 784.0 mg

HCl (concentrated) 5.0 ml

CoCl2 x H2O 238.0 mg

(NH4)2Ni(SO4)2 x 6 H2O 395.0 mg

Na2MoO4 x 2 H2O 24.0 mg

Na2WO4 x 2 H2O 33.0 mg

ZnSO4 x 7 H2O 144.0 mg

CuCl2 x 2 H2O 2.0 mg

Na2SeO4 94.0 mg

H3BO3 6.0 mg

MnCl2 x 4 H2O 99.0 mg

Distilled water 995.0 ml

Mohr's salt is dissolved firstly in concentrated HCl and mixed with distilled water and other salts are dissolved in the sequence indicated.


Vitamin solution:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine 0.1 mg

Riboflavin 50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitamin B12 1.0 mg

Lipoic acid 50.0 mg

Distilled water 1000.0 ml

The medium had a pH of 6.5–6.8 (measured at 25°C). The medium was dispensed in 10 ml portions into 17 ml Hungate tubes and heat-sterilized at 121°C for 30 min; the gas phase consisted of H2/CO2 (20 : 80 v/v). Sodium sulfite from a sterile anoxic stock solution was added as an electron acceptor to a final concentration of 5 mM.

Updated: 08.01.2024