90. DESULFOVIBRIO GIGAS MEDIUM

Solution 1:

KH2PO4 1.0 g

NH4Cl 0.5 g

MgSO4 7 H2O 0.4 g

Na2SO4 2.0 g

CaCl2 2 H2O 0.1 g

Trace element solution SL-6: (see below) 1.0 ml

2 M H2SO4 1.0 ml

Na L-lactate 2.0 g

Distilled water 950.0 ml

Solution 2:

NaHCO3 2.0 g

Distilled water 40.0 ml

Solution 3:

Na2S 9 H2O 300.0 mg

Distilled water 6.0 ml

Vitamin solution:

Biotin 2.5 mg

Nicotinic acid 25.0 mg

Thiamine-HCl 12.5 mg

p-Aminobenzoic acid 12.5 mg

Ca-pantothenate 6.5 mg

Pyridoxine-HCl 62.5 mg

Distilled water 1000.0 ml

Trace element solution SL-6:

ZnSO4 7 H2O 0.1 g

MnCl2 4 H2O 0.03 g

H3BO3 0.3 g

CoCl2 6 H2O 0.2 g

CuCl2 2 H2O 0.01 g

NiCl2 6 H2O 0.02 g

Na2MoO4 0.03 g

Distilled water 1000.0 ml

pH 7.2

Autoclave solutions 1 and 3 separately under N2 at 121C for 15 min, add 5.0 ml of the filter sterilized vitamin solution to 1000.0 ml of sterile solution 1. Solution 2 (autoclave at 121C for 15 min) is not to be kept for long.

 

Updated 09.08.2021