90. DESULFOVIBRIO GIGAS MEDIUM

Solution 1:

KH₂PO₄ 1.0 g

NH₄Cl 0.5 g

MgSO₄ × 7 H₂O 0.4 g

Na₂SO₄ 2.0 g

CaCl₂ × 2 H₂O 0.1 g

Trace element solution SL-6: (see below) 1.0 ml

2 M H₂SO₄ 1.0 ml

Na L-lactate 2.0 g

Distilled water 950.0 ml

Solution 2:

NaHCO₃ 2.0 g

Distilled water 40.0 ml

Solution 3:

Na₂S × 9 H₂O 300.0 mg

Distilled water 6.0 ml

Vitamin solution:

Biotin 2.5 mg

Nicotinic acid 25.0 mg

Thiamine-HCl 12.5 mg

p-Aminobenzoic acid 12.5 mg

Ca-pantothenate 6.5 mg

Pyridoxine-HCl 62.5 mg

Distilled water 1000.0 ml

Trace element solution SL-6:

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

H₃BO₃ 0.3 g

CoCl₂ × 6 H₂O 0.2 g

CuCl₂ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

pH 7.2

Autoclave solutions 1 and 3 separately under N₂ at 121°C for 15 min, add 5.0 ml of the filter sterilized vitamin solution to 1000.0 ml of sterile solution 1. Solution 2 (autoclave at 121°C for 15 min) is not to be kept for long.

 

Updated 01.02.2024