91. THERMODESULFOBACTERIUM MEDIUM

Solution 1:

Na2SO4 3.0 g

NH4Cl 1.0 g

MgCl2 6 H2O 0.2 g

KH2PO4 0.3 g

Na2HPO4 12 H2O 2.0 g

FeSO4 7 H2O 1.5 mg

Resazurin 1.0 mg

Distilled water 930.0 ml

Solution 2:

Trace element solution (see below) 10.0 ml

Solution 3:

Yeast extract 1.0 g

Distilled water 25.0 ml

Solution 4:

Na-lactate 4.0 g

Distilled water 25.0 ml

Solution 5:

Na2S 9 H2O 0.5 g

Distilled water 6.0 ml

Solution 6:

Vitamin solution (see below) 5.0 ml

Trace element solution:

Nitrilotriacetic acid 12.8 g

FeCl2 4 H2O 0.2 g

MnCl2 4 H2O 0.1 g

CoCl2 6 H2O 0.17 g

CaCl2 2 H2O 0.1 g

ZnCl2 0.1 g

CuCl2 0.02 g

Na2MoO4 2 H2O 0.01 g

NiCl2 6 H2O 0.026 g

NaCl 1.0 g

Na2SeO3 5 H2O 0.02 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

Ca DL-pantothenate 5.0 mg

Vitamin B12 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

pH 6.8-7.0

Solutions 1, 3, 4 and 5 autoclave under N2 at 121C for 15 min.

Solution 6 is filter sterilized. Before use, neutralize solution 5 by dropwise of 1 N HCl.

 

Updated 21.06.2021