94. MEDIUM FOR CARBON MONOOXIDE OXIDIZERS

Na2HPO4 12 H2O 4.5 g

KH2PO4 0.75 g

NH4Cl 1.5 g

MgSO4 7 H2O 0.2 g

CaCl2 2 H2O 0.03 g

Fe(NH4)-citrate 0.018 g

Agar (if necessary) 1.2 g

Trace element solution SL-6 (see below) 1.0 ml

Vitamin solution (see below) 10.0 ml

Distilled water 1000.0 ml

Trace element solution SL-6:

ZnSO4 7 H2O 0.1 g

MnCl2 4 H2O 0.03 g

H3BO3 0.3 g

CoCl2 6 H2O 0.2 g

CuCl2 2 H2O 0.01 g

NiCl2 6 H2O 0.02 g

Na2MoO4 0.03 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl 2 H2O 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

D-Ca-pantothenate 5.0 mg

Vitamin B12 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121C for 15 min, add vitamin solution sterilized by filtration. For chemoautotrophic growth incubate under gas atmosphere of a) 20-80% CO2 + 10% O2 + 0-70% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.5 g NaHCO3 per liter of medium. For chemoorganotrophic growth add 3.0 g sodium acetate and incubate under air.

 

Updated 09.08.2021